Mouse Osteoblast Cell Sensitivity to the AC Magnetic Field at 14 Hz

Since the surface electrical current of the attached mouse osteoblast cell monolayer in a culture plate could induce magnetic fluctuation, power density spectrum of the geomagnetic fluctuation (GF) recorded at the sample rate 2000 times per second illustrated the signal to noise ratio (SNR) spectrum in a frequency interval (0 ~ 60 Hz) for the mouse osteoblast cells system. In contrary to the background, 14±2 Hz was an intrinsic signal within cells. We exposed cells into a constant extremely low frequency (ELF) magnetic field (~ 50 m Gauss) at 14 Hz for 60 minutes, 20% modulation of the gap junction intracellular communication (GJIC) within mouse osteoblast cells was observed from the analysis of Lucifer yellow fluorescence microscopic image while comparing with the control group. Cellular response, GJIC modulation caused by the ELF magnetic field applied through ac function generator and solenoid coil was relied on the resonance within cells at intrinsic frequency. [Nature and Science 2003;1(1):27-31].